Expression and Bioinformatics Analysis of Tasp-Spag1 Genes of Theileria annulata

To explore the characteristics of the Theileriaannulatasurface antigens,the surface antigen Tasp-Spag1 genes were co-expressed in prokaryotic expression system.To bioinformatically analyze the surface antigen Tasp-Spag1 genes.Tasp-Spag1 genes were amplified by PCR method to construct recombinant plasmid of pET-28a-Tasp-Spag1.After the inducible expression by IPTG,SDS-PAGE,Western blot analyses were used.Bioinformatics software was used to analyze the Tasp-Spag1 genes,the main characters and antigenic epitopes were predicted.Tasp-Spag1 genes were about 729 bp amplified by PCR,then,the result of SDS-PAGE,Western blot showed that the target protein was obtained with a molecular weight same as the expected size;Based on the bioinformatics analysis,the recombinant Tasp-spag1 contains 236amino acids and belongs to unstable hydrophilic nonsecreted non-transmembrane protein.It contains 33 glycosylation sites and 21 phosphoric sites,3low complexity domain and Sorb,NL homologous regions.There may be 10 B-cell major epitope domains and 7 T-cell major epitope domains and two cross-reactive epitopes.The predicted results indicated that two different recombinant proteins have good immunogenicity in theory.