高危人乳头瘤病毒58型E6基因的克隆及表达
Journal of the Fourth Military Medical University(2004)
Abstract
目的:获得含人乳头瘤病毒(HPV)58型E6基因的克隆及表达重组体并体外表达E6蛋白. 方法:聚合酶链方法从1例宫颈腺癌患者癌组织DNA中获得HPV58 E6基因,并将其与克隆载体pGEM-T Easy连接,获得重组体HPV58-E6-pGEM-T,继之以双酶切将E6基因与同样双酶切的线性化的pRSET-A表达载体连接,得到E6表达重组体pRSET-58E6,转化E.coli BL21(DE3),用IPTG诱导表达. 结果:从1例宫颈癌患者中成功获得了少见HPV58型的E6基因并构建了其重组表达载体. 经IPTG诱导后可表达Mr 24 000的6His HPV58 E6融合蛋白,表达量占菌体蛋白的10%. 结论:成功获得了少见HPV58高危型的E6基因,并可在E.coli中高效表达.
MoreTranslated text
Key words
human papillomavirus type,e6 gene,cloning
AI Read Science
Must-Reading Tree
Example
![](https://originalfileserver.aminer.cn/sys/aminer/pubs/mrt_preview.jpeg)
Generate MRT to find the research sequence of this paper
Chat Paper
Summary is being generated by the instructions you defined