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普通野生稻中NBS同源序列的克隆和分析

Zhou Weibin,Shi Xi,Zhan Shu-xuan,Sun Chong-rong,Cao Kai-ming

Journal of Fudan University（2001）

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Abstract
已知的植物抗病基因大多数具有核苷酸结合位点(NBS)和富含亮氨酸重复区(LRR).根据 NBS的保守区设计引物,应用聚合酶链式反应(PCR),从普通野生稻基因组中克隆了4个不同的NBS同源序列(OSNBA1～OSNBA4).同源性比较显示它们均与非TIR类的NBS序列相似.其中,OSNBA1与双子叶植物拟南芥的RPS2基因更接近;OSNBA2～OSNBA 4与单子叶植物水稻的XA1、RPR1基因更相似.
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Key words
nucleotide binding site homologues,molecular cloning
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