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蕲蛇蛇毒中一个新的类凝血酶的分离纯化与表征

Life Science Research(2002)

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Abstract
使用DEAE-Sephadex A-50、POROS 50HS及POROS 20PI等色谱分离技术,从蕲蛇粗毒中分离提纯得到1个具有凝血活力的组分,经SDS-PAGE和PAGE检测均为一条带,非还原条件下相对分子质量24.3kDa,还原条件下相对分子质量33.0kDa;其凝血活力为91.0 NIH u/mg.该组分不具有激活因子ⅩⅢ的活性,肝素不影响该组分的凝血活性,EDTA部分抑制其活性,苯甲基磺酰氟(PMSF)则会产生不可逆抑制作用.该酶N-末端氨基酸序列为VIGGNGXDINEHRFLVAFF,经分析表明该酶是一种新的类凝血酶.
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Key words
enzyme,purification,thrombin-like
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