Protective Effect of Ozone Preconditioning on Renal Fibrosis After Ischemia-Reperfusion Injury in Rat

Objective:To investigate the protective effect of ozone preconditioning on renal fibrosis after ischemia-reperfusion injury in rat and its mechanisms.Methods:Twenty-four male Wistar rats were randomly divided into 3groups as sham operation group,ischemia-reperfusion group,and ozone preconditioning group.Renal and serum samples were collected.The concentration of serum creatinine and blood urea were detected 8weeks later.And after HE staining,the pathological changes in the left kidneys of rats were observed under the light microscope.The expressions ofα-SMA,TGF-β1and Smad7 were detected by Realtime PCR and Western blot.Results:There was no statistical difference in the renal function among the three groups(P0.05);More renal damages could be observed in the ischemia-reperfusion group than in the ozone pre-treatmentgroup.Ozone preconditioning could obviously alleviate the renal injury induced by ischemia and reperfusion injury.The test results of Western blot and Realtime PCR showed that the expressions ofα-SMA and TGF-β1were higher in the ischemia-reperfusion group than in the ozone preconditioning group(P0.05).And the expression of Smad-7was higher in the ozone preconditioning group than in the ischemia-reperfusion group(P0.05).Conclusion:The ozone preconditioning is the protective factor to the renal fibrosis after ischemia reperfusion injury in rat,and its mechanism is possibly related to the expressions of TGF-β1and Smad 7.