Effects and Mechanism of Trichostatin A on the Collagen Induced Arthritis Mice

Objective:To investigate the effects and mechanism of Trichostatin A(TSA)on the mice model of collagen induced arthritis(CIA).Methods:The DBA/1male mice were randomly divided into model group,TSA group and control group.At the end of 34 days after the injection,the mean arthritic index,the synovitis score and the histopathologic examination were evaluated on the mice.The mRNA and protein expression of IFN-γ,IL-17,and IL-4were detected by realtime PCR and ELISA.Results:The MAI of the TSA group(2.81±0.10)was remarkably lower than that of the model group(3.63±0.23).The mRNA expression of IFN-γ,IL-17 of the TSA group(6.39±0.51,2.94±0.40)were lower than that of the model group(7.99±0.63,5.87±0.24),while IL-4of the TSA group(2.11±0.15)was higher than that of the model group(1.12±0.07),P0.05.Conclusion:TSA might mitigate the disease of CIA by inhibiting Th1 and Th17cells secreting cytokine IFN-γand IL-17 as well as increasing Th2 cells secreting cytokine IL-4.