Fermentation coupled with high concentration extraction for Nisin production

The experiment mainly adopts the material after corn deep processing to fermented. The fermentation medium includes glucose, protein and maize lixivium, also equips with yeast extract, sugar cane, inorganic salt and biotin at the same time. After the fermentation, fermented liquid through acidification and aeration to highly concentrated and purification. Then through organic membrane to purify soluble impurities and salts. Using spray drying to gain the Nisin finished product, and in the product Nisin titer can reach 8000～15000 IU/mL.