Comparative Analysis in Molecular Characteristic of the Oct-4 expressing Cells Isolated from Sheep Amniotic Fluid and Fetal Renal Tissues

This study aimed at developing a novel technique of isolating the Oct-4expressing cells from the sheep amniotic fluid and fetal renal tissues,respectively,and comparatively analyzing the difference in the molecular characteristic of these two differently derived cells with Real-time PCR.The results showed that the cells from the sheep amniotic fluid and fetal renal tissue were both positive for Oct-4,embryonic germ cells gene SSEA-1,the main organization compatibility antigen gene MHC-Ⅱ,apoptosis gene Bax,and inhibition gene of apoptosis Bcl-2,negative for the symbol marker of MHC-Ⅰ,Nanog and TERT,but there were marked differences in the relative expression levels of Oct-4,Baxand Bcl-2genes.Furthermore,AFSC and RTSC were both capable of forming embryoid bodies through the suspension culture in vitro.In conclusion,our study successfully established a new procedure of isolating and culturing Oct-4expressing cells derived from the sheep amniotic fluid and fetal renal tissues,which might have the same origin.