菜薹小孢子培养诱导胚状体的影响因素研究
Hunan Agricultural Sciences(2013)
Abstract
以8个不同基因型菜薹品种为试材,对影响小孢子培养出胚率的主要因素及胚状体植株再生的条件进行了研究.结果表明:材料的基因型对出胚的影响很大,红菜薹以H09-1的出胚率最高,为111个胚/蕾,白菜薹以B09-1最高,为0.514胚/蕾;最适宜的小孢子培养浓度为2×105个/mL;菜薹小孢子培养最佳的胚状体诱导培养基激素组合为1.0 mg/L 6-BA+0.4 mg/L NAA;胚状体植株成苗率最高的培养基琼脂含量以8~10 g/L为宜.
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Key words
brassica campestris,microspore culture,embryoid induction
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