叶原基形态对甘薯茎尖脱毒培养的影响
Journal of Southwest University(2014)
Abstract
以带有甘薯羽状斑驳病毒(SPFMV)的渝薯2号,从其块根萌芽上剥取2叶原基形态分别处于突起态、半闭合态和闭合态0.22~0.35 mm大小的茎尖为外植体,培养于附加BAP与IAA,BAP与NAA不同激素体积质量分数组合的MS培养基上进行成苗培养,对应再生株系用硝酸纤维素膜酶联免疫吸附检测法(NCM ELISA)进行病毒检测.结果表明:茎尖培养最佳的激素体积质量分数组合为0.8~1.4 mg/L BAP+0.01 mg/L NAA,有利于茎尖基部发生少量的愈伤组织,芽粗壮且成苗快.成苗率因外植体而不同,闭合态和半闭合态较高,分别达92.9%和88.9%,而突起态成苗率仅为46.7%.脱毒效果也与外植体相关,最佳效果是叶原基突起态,其次是半闭合态和闭合态,分别获得100%,83.9%和71.9%的脱毒植株.
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Key words
leaf primordium,virus-free
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