The feasibility of identification of para-Bombay blood group with PCR-SSP technology(a report of two cases)

Objective To evaluate the feasibility of rapid identification of ABO blood group for para-Bombay phenotype using PCR-SSP technology. Methods The four pairs of special specific primers were designed according to the cDNA nts261,703 of A101 allele with Primer 5 software. Two cases of suspected para-Bombay phenotype were detected using the PCRSSP,besides,the Exon6 and Exon7 of ABO gene and FUT1 gene were sequenced by the Sequencing Technology. Results The genotype of ABO blood group obtained from PCR-SSP was B /B,A /O respectively,and the exact genotype showed B101 /B101,A101 /O01 by sequencing technology respectively; The results indicated that one homozygous mutation was detected by direct sequencing in case 1: AG deletion at position 547-552( AGAGAG→AGAG). Two heterozygous mutations were detected in case 2: AG deletion at position 547-552 and C to T mutation at position 658. Conclusion The PCR-SSP technology coan discriminate the genotype of ABO blood group gene rapidly for the suspected para-Bombay phenotypes,which work as an independent method to offset the defect of serological method,eventually improve the accuracy of identification of ABO blood group for the suspected para-Bombay phenotypes