Simultaneously Determination of Aesculetin and Caffeic Acid in Compound Dandelion Granule by HPLC

In order to improve the quality standard of compound dandelion granule,an HPLC method was established for simultaneously determination of aesculetin and caffeic acid in compound dandelion granule. The isolation was performed on a Wonda Cract ODS- 2 column( 4. 6 mm × 250 mm,5 μm) with mobile phase consisting of methanol – 0. 6% glacial acetic acid( 20 ∶ 80). The flow rate was 1. 0 m L / min,the detection wavelength was 326 nm and the column temperature was 25 ℃. The results showed that good linear relationship was obtained in the range of 0. 02 ~ 0. 32 mg / m L and 0. 004 ~ 0. 064 mg / m L respectively( R2= 0. 9999). The average recoveries of aesculetin and caffeic acid were 100. 08% and 99. 69% with RSD of 0. 98% and 0. 84%.The method was accurate,sensitive and reliable with a good reproducibility and can be used as an quantitative analysis of aesculetin and caffeic acid in compound dandelion granule.