Effects of Yiqi Huayu Jiedu method on EGFR / MAPK signal pathway in rats with chronic atrophic gastritis exhibiting dysplasia

Objective To observe the effects of Yiqi Huayu Jiedu method on EGFR / MAPK signal pathway in rats with chronic atrophic gastritis exhibiting dysplasia. Methods 60 Wistar rats were randomly divided into model group( n = 50) and blank control group( n = 10). The blank control group rats were given standard SPF animal diet throughout the study. Three measures were combined to establish model of chronic atrophic gastritis with dysplasia,including intragastric administration of 120μg /m L MNNG,5 m L /kg,1 time /d,assisted by 0. 1% ammonia drinking water and 0. 03% ranitidine in feed. After the model was successfully established at the 28 th week,30 modeled rats were randomly divided into model group,vitacoenzyme group and Yiqi Huayu Jiedu group,with 10 rats in each group.Each group was given the corresponding medicine once daily: 3 m L / kg saline for the model group,2m L / kg vitacoenzyme suspension liquid( 0. 3 g / kg vitacoenzyme) for the vitacoenzyme group and 3 m L /kg herbal solution( containing 9 g / kg crude herb). All the rats were sacrificed after 12 weeks. The method of Western blot was used for measuring the protein expression of EGFR,ERK1 /2,and p-ERK1 /2 in rats of all groups. Results The EGFR protein expression of the model group was higher than that in the vitacoenzyme group,the Yiqi Huayu Jiedu group,and the blank group( P = 0. 000); it was significantly lower in Yiqi Huayu Jiedu group than the model group( P = 0. 000) and also lower than vitacoenzyme group( P 0. 05). Compared with the blank control group,there was no difference( P 0. 05). The ERK1 /2 protein expression of the model group was higher than that in the vitacoenzyme group,the Yiqi Huayu Jiedu group,and the blank group( P = 0. 000) in a descending order; it was significantly lower in Yiqi Huayu Jiedu group than the model group and vitacoenzyme group( both P = 0. 000) and significantly higher than the blank group( P 0. 01). The p-ERK1 /2 protein expression of the model group was significantly higher than that in the blank group( P = 0. 000); it was significantly lower in Yiqi Huayu Jiedu group than the model group and vitacoenzyme group( both P = 0. 000). Compared with the blank group,there was no significant difference( P 0. 05). Conclusion Yiqi Huayu Jiedu method could reverse gastric mucosal dysplasia. Its mechanism of action could be reducing high expression of EGFR protein and blocking abnormal activation of the EGFR / MAPK cellular signal transduction pathway.