HPLC指纹图谱分析血清中四逆散冻干粉的相似度
The Chinese Journal of Clinical Pharmacology(2015)
Abstract
目的:研究四逆散冻干粉血清的高效液相色谱指纹图谱的相似度。方法用HPLC方法,色谱条件:Kromasil C18柱(4.6 mm ×250 mm,5μm),流速1.0 mL? min-1,柱温30℃,流动相A为乙腈;流动相B为pH=3磷酸水,流动相A梯度洗脱(10%~90%乙腈),分析时间为150 min,采用高效液相指纹图谱方法,分析10批四逆散冻干粉血清样品。结果10批四逆散冻干粉血清样品,在240 nm处获得比空白血清多8个共有峰,四逆散冻干粉一次给药5 h后,产生了8个血中移行成分。相似度分析结果均在0.99以上。结论四逆散冻干粉血清的指纹图谱特征性及专属性强。
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Key words
Sinisan lyophilized power,serum,HPLC,fingerprinting
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