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两种不同方式介导IL-12真核表达载体抑制肝移植瘤生长的研究

Chinese Journal of Current Advances in General Surgery(2004)

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Abstract
目的:比较两种不同方式介导IL-12真核表达载体抑制肝移植瘤生长的效果.方法:利用鼠肝癌细胞系H22建立肝移植瘤模型,以脂质体介导IL-12质粒腹腔注射或直接瘤内注射给荷瘤鼠,每周1次,共2次.治疗后每周两次测量瘤体大小,取血利用ELISA法检测血清中IL-12及IL-2的含量变化;MTT法检测NK细胞杀伤活性.结果:直接瘤内注射组瘤体积明显小于腹腔注射组(P<0.05),直接瘤内注射组血清IL-12、IL-2浓度有明显的周期变化,但二者NK细胞杀伤率无明显差异.结论:脂质体介导直接瘤内注射IL-12真核表达载体可有效抑制肝移植瘤的生长.
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Key words
transplanted liver cancer,eukaryotic expression vector
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