QTL Mapping of Seed Amino Acids Content in Soybean

A cross of Jidou 12 and Heidou was made to construct a recombinant inbred line of F8 population which has 186 progenies as the experimental material. Correlation between 16 amino acid content were analysis in Shijiazhuang environment.ANOVA,CIM and ICIM were used to identify quantitative trait loci. The results showed that the distribution of 16 traits was continuous and normal distribution. Fourteen QTL were detected across three method about glutamic acid,arginine,serine,alanine,phenylalanine,isoleucine,aspartic acid and lysine,distributed at B1,D1 a,D1b,H,I,J and K linkage group,contribution rate from 4. 91% to 17. 02%. q Phe-20-1 had the highest contribution rate( 17. 02%),detected by ICIM,favorable alleles originate from Heidou. There were three QTL q Asp-20,q Glu-20 and q Lys-20 detected by CIM in the same marker interval with q Phe-20-1. q Arg-11 was detected by ANOVA and ICIM,contribution rate was 11. 79%,favorable alleles originate from Jidou 12. These results provide a theoretical basis for marker-assisted breeding and fine mapping.