Determination of 1,3- dideoxygalactonojirimycin from bombycis faeces

Objective: The paper establishes a simple and rapid method for the determination of 1,3-Dideoxygalactonojirimycin from bombycis faeces by HPLC. 1,3- dideoxygalactonojirimycin in bombycis faeces is a potent glucosidase inihibitor. Methods: A RP- HPLC method with fluorescence detection has been used. Results: The HPLC method in this research has a good reliability including accuracy and precision. The detection limit was less than 72 ng. Conclusion This method is sufficiently sensitive for determining 1,3- dideoxygalactonojirimycin in bombycis faeces and other related products.