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正交试验法优化吴茱萸水提工艺

魏宁,刘庆元

Chinese Journal of Information on TCM（2004）

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Abstract
目的用正交实验提取吴茱萸中有效成分吴茱萸次碱,优选吴茱萸的工业提取路线.方法采用HPLC法测定吴茱萸水提物中吴茱萸次碱的含量,测定结果进行方差分析,求出最佳条件.结果实验表明,煎煮次数、溶媒用量对吴茱萸次碱的提取影响较大,煎煮时间则次之.结论经筛选,吴茱萸中生物碱水提取的工艺路线为:煎煮0.5 h,煎煮3次,每次用16倍量水,在此条件下吴茱萸中生物碱提取完全.
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