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携带小鼠CD40Ig基因重组腺病毒的构建及鉴定

夏天,苟兴华,严律南,李德华,胡海洋,闫娟,李波,刘忠荣

Chinese Journal of General Surgery（2004）

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Abstract
目的构建携带小鼠CD40Ig基因的重组腺病毒载体,为研究供体特异性移植耐受诱导做准备.方法分别克隆小鼠CD40胞外区cDNA和IgG2a-Fc段cDNA,以PCR方法将二者连接为CD40Ig.再将后者装入腺病毒穿梭质粒pAdTrack-CMV,与骨架质粒在大肠杆菌BJ5183中同源重组,经293细胞包装、扩增后得到携带CD40Ig的重组腺病毒AdCD40Ig.结果成功构建携带CD40Ig的重组腺病毒载体系统,病毒滴度为5×109efu/ml.结论构建的AdCD40Ig可用于供体特异性移植耐受的实验研究.
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Key words
adenovirus vector,gene
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