The effect of anodised treatment on zirconium implant at early stages: A preliminary in vivo and in vitro characterization

Event Abstract Back to Event The effect of anodised treatment on zirconium implant at early stages: A preliminary in vivo and in vitro characterization Maria Katunar1, Andrea Gomez-Sanchez1, Juliana Pellejero1, Josefina Ballarre1, Ana Santos-Coquillat2, Enrique Martinez-Campos2, Ana Civantos3, Matias Baca4, Carlos B. Vottola4, Khalil Haddad4, Viviana Ramos2 and Silvia Cere1 1 INTEMA. Universidad Nacional de Mar del Plata (UNMdP)-CONICET, Division Corrosion, Argentina 2 Intituto de Estudios Biofuncionales. Universidad Complutense de Madrid, Spain 3 Instituto de Ciencia y Tecnología de Polímeros, CSIC., Spain 4 Hospital Interzonal Generalde Agudos (HIGA) Oscar Alende, Mar del Plata., Traumatología y Ortopedia, Argentina Most metals used as cementless implants undergo some kind of surface modification before clinical insertion. These modifications are performed to promote biological reactions at the interface influencing principally in the biological events that lead to bone formation.Cementless prosthesis were principally the primary choice for young patients[1][2]. A variety of surface modifications have been studied and applied to implants to achieve long-term fixation to the host bone by osseointegration. The texturing and /or chemical alterations of material surfaces may lead to long-term integration in bone, so implant topography is critical to the success of bone-anchored implants[3][4][5]. Zirconium (Zr) is an ideal metal for intra-osseous implants for its favorable resistance to corrosion, osseointegration capability and lower metal ions migration to the biological surroundings when it is compared with stainless steel and titanium alloys. Zr and its alloys have been studied for being used in the nuclear power industry and have been recently commercialized for its use in medical implants, especially for total knee and hip replacements. The aim of this study is focused on the first events that take place around anodized Zr implants. The surface in vitro cytocompatibility was evaluated by observing cell adhesion, proliferation, and differentiation using pre mioblastic C2C12 cell line.This cell line was treated with a lentivirus with Green Fluorescent Protein (GFP) allowing cell visualization over opaque biomaterials, showing an optimum growth and cell behavior. Our in vitro results highlight that anodisation treatment at 60V allowed stronger cell attachment promoting cell proliferation at early stages. Evaluation of cell morphology through fluorescent microscopy showed an interesting relationship between cell differentiation and material surface. In vivo implant osseointegration was evaluated by histomorphometrics characteristics of new bone formation. Male Wistar rats were employed and the implants Zr0 (without treatment),Zr30 (with anodized 30V) and Zr60(with anodized 60V) were placed by press fit into femur extending into the medullar canal. The biological events were evaluated 15 and 30 days after surgery. These results showed that Zr implants had an optimal new bone density distribution, and optimal bone–implant osseointegration 15 and 30 days after surgery. The thickness of the new bone showed that anodized at 60V treatment improved notoriously the characteristic of the new bone growth just 15 days after surgery. The profile of bone formation was analyzed by polychrome fluorescent labeling using calcium-binding fluorochromes and the results showed that bone thickness is accompanied by an significant increased in MAR parameter at early stages. In conclusion, it can be speculated that coupling the positive results obtained both in vitro and in vivo conditions of anodisation treatment on Zr features can be beneficial for future orthopedic applications. Josefina BallarreReferences:[1] So K. et al. " Good short-term outcome of primary total hip arthroplasty with cementless bioactive glass ceramic bottom-coated implants: 109 hips followed for 3–9 years".. Acta Orthop. 2012 Dec;83(6):599-603.[2] Harumoto Yamada et al." Cementless total hip replacement: past, present, and future" J Orthop Sci (2009) 14:228–241[3] Hoerth, R.M et al."A comparative study of zirconium and titanium implants in rat: Osseointegration and bone material quality” Journal of Materials Science: Materials in Medicine. Volume 25, Issue 2, February 2014, 411-425[4] Maria R. Katunar et al. ""Anodised Zirconium as a possible candidate for endosseous implants: A preliminary electrochemical and in vivo approach" Progress in Biomaterials (2014) 3:24-29.[5] Sul YT.et al." Characteristics of the surface oxides on turned and electrochemically oxidized pure titanium implants up to dielectric breakdown: the oxide thickness, micropore configurations surface roughness, crystal structure and chemical composition." Biomaterials. 2002 Jan;23(2):491-501 Keywords: Bone Regeneration, in vivo, Implant, Cell response Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biocompatibility of nanobiomaterials Citation: Katunar M, Gomez-Sanchez A, Pellejero J, Ballarre J, Santos-Coquillat A, Martinez-Campos E, Civantos A, Baca M, Vottola CB, Haddad K, Ramos V and Cere S (2016). The effect of anodised treatment on zirconium implant at early stages: A preliminary in vivo and in vitro characterization. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00871 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. 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Abstract Info Abstract The Authors in Frontiers Maria Katunar Andrea Gomez-Sanchez Juliana Pellejero Josefina Ballarre Ana Santos-Coquillat Enrique Martinez-Campos Ana Civantos Matias Baca Carlos B Vottola Khalil Haddad Viviana Ramos Silvia Cere Google Maria Katunar Andrea Gomez-Sanchez Juliana Pellejero Josefina Ballarre Ana Santos-Coquillat Enrique Martinez-Campos Ana Civantos Matias Baca Carlos B Vottola Khalil Haddad Viviana Ramos Silvia Cere Google Scholar Maria Katunar Andrea Gomez-Sanchez Juliana Pellejero Josefina Ballarre Ana Santos-Coquillat Enrique Martinez-Campos Ana Civantos Matias Baca Carlos B Vottola Khalil Haddad Viviana Ramos Silvia Cere PubMed Maria Katunar Andrea Gomez-Sanchez Juliana Pellejero Josefina Ballarre Ana Santos-Coquillat Enrique Martinez-Campos Ana Civantos Matias Baca Carlos B Vottola Khalil Haddad Viviana Ramos Silvia Cere Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. 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