Gentamicin-induced Acute KidneyInjury in Equines is associated withMarked Acute Phase Response: AnExperimental Study on Donkey(Equus asinus)

Gentamicin-induced Acute Kidney Injury in Equines is associated with Marked Acute Phase Response: An Experimental Study on Donkey (Equus asinus)Recently, there is growing evidence suggesting that acute kidney injury (AKI) in human and laboratory animals is associated with an inflammatory response that could play a role in tissue damage. However, such link has not previously been addressed in equines. The present study was designed to evaluate the effect of gentamicin (GEN) administration on the development of AKI and systemic inflammatory response in equines using donkey as a model. GEN (10%) was administered intravenously in six donkeys at a dose of 20 mg kg-1 BW thrice daily for 14 consecutive days. Three other donkeys were randomly assigned to receive saline solution and served as controls. The donkeys were clinically and sonographically examined throughout the experimental period. Blood and urine (U) samples were simultaneously collected at day (D) 7, and D 14 of GEN administration. Renal specimens from all donkeys were collected at D 14 and processed for routine histopathological examination. AKI was confirmed by sonography, laboratory measurements, histopathology and immunohistochemistry.