Tu1734 Acupuncture for the Treatment of Functional Dyspepsia: Systematic Review and Meta-Analysis

in microbiota-interaction was calculated for CD and UC.16S rDNA paired-end sequencing targeting the V4 hypervariable region was performed using Illumina MiSeq sequencer.Sequencing depth was downsized to 10000 reads/sample.The Ribosomal Database Project classifier was used for taxonomic assignment.Statistical analyses were performed with R, using parametric and non-parametric tests, with multiple testing correction (FDR).Correlation between genera abundances and GRS was performed with Spearman correlation.Results: Although microbial richness did not differ between patients with a high (n=26) or low (n= 26) PSC GRS, and no differences were observed in the abundance of specific phyla, genera, or operational taxonomic units (OTU), the overall microbiota composition was significantly different between the 2 groups (Adonis test on Bray-Curtis dissimilarity, p-value 0.002).Interesting to note, the abundances of 9 genera were significantly different between the 2 groups before multiple testing correction, including Prevotella, Dorea, Methanosfera, and Paraprevotella.No significant association was observed between CD or UC GRS quartiles and microbiota composition, richness, or taxa abundances in this cohort.Conclusion: The genetic risk for PSC seems to influence the overall gut microbiota composition in patients with PSC, however no specific significant differences in microbial diversity or differences of OTU, genus or phylum abundances were observed between patients with high and low GRS for PSC.The cumulative effect of multiple small differences at genus level may explain the overall significant difference in community composition.Larger sample size may be needed to evidence significant taxon abundance associations to GRS.Contrarily to PSC GRS, the CD or UC GRS do not seem to influence intestinal microbiota in this population.