Aligned melt spun PCL fibers orientate smooth muscle cells and the preservation of the contractile phenotype

Event Abstract Back to Event Aligned melt spun PCL fibers orientate smooth muscle cells and the preservation of the contractile phenotype Animesh Agrawal1, Bae Hoon Lee1, Scott A. Irvine1, An Jia2, Ramya Bhuthalingam1, Vaishali Singh3, Kok Yao Low4, Chee Kai Chua2 and Subbu S. Venkatraman1 1 Nanyang Technological University, School of Material Science & Engineering, Singapore 2 Nanyang Technological University, School of Mechanical & Aerospace Engineering, Singapore 3 Indian Institute of Technology, Department of Polymer & Process Engineering, India 4 Nanyang Technological University, School of Biological Sciences, Singapore Introduction: The cell seeding of a small diameter vascular prosthesis is widely believed to be a worthwhile goal. Contractile smooth muscle cells (SMCs) seeded circumferentially on a vascular prosthesis will give a vasoactive component to the construct[1]. Contractile SMCs allows the changes that mediate blood pressure by altering the lumen diameter. However, SMCs have phenotype plasticity between contractile and synthetic, and SMCs frequently lose the useful contractile phenotype during culturing. The elongated orientation of SMCs along parallel grooves preserves the contractile phenotype for longer than seeding on an unpatterned surface[2]. Polycaprolactone (PCL) fibers can be melt spun onto a mandrel, and then bonded in place using 5% chitosan solution, thus producing an aligned fiber surface, presenting parallel grooves 10µm wide and 10µm deep. This could be fabricated into a 2D fiber mat or deposited circumferentially on a proposed PCL tubule vascular prosthesis. Methods: PCL was melt spun onto a rotating mandrel, producing a layer 1 fiber deep. The fibers were dip coated into 5% chitosan solution to adhere them in place. The fibers were presented as a 2D fiber map and seeded with SMCs. Phenotype of the SMCs were assessed using immunofluorescence staining for α smooth muscle actin. The fibers were deposited onto a 5mm diameter potential vascular conduit to assess whether SMC could be seeded and circumferentially orientated onto the tube. Results and Discussions: SMCs seeded onto the melt spun fibers assumed an elongated morphology in the direction of the melt spun fibers, whereas SMCs seeded on an unpatterned surface were more rhomboid in appearance. The cells on the aligned fibers retained the contractile αSMA marker for up to 7 days whereas the signal was lost in the cells on the unpatterned surface by 3 days. When applied to a 5mm conduit tube, the melt spun PCL fiber supported SMC adhesion to the surface and orientated the cells to align circumferentially in the direction of the fibers. Hence meltspun PCL fibers demonstrated here can be applied to a tubular vascular prosthesis to promote the retention of the desired vasoresponsive contractile phenotype. Prof. Mark Featherstone; Singapore National Research Foundation; A∗STAR, SERC, Public Sector Research Funding (PSF)References:[1] R.G.Thakar, F.Ho, N. F. Huang, D. Liepmann, and S. Li, “Regulation of vascular smooth muscle cells by micropatterning,” Biochemical and Biophysical Research Communications, vol. 307, no. 4, pp. 883–890, 2003[2] Y. Cao, Y. F. Poon, J. Feng, S. Rayatpisheh, V. Chan, and M. B. Chan-Park, “Regulating orientation and phenotype of primary vascular smooth muscle cells by biodegradable films patterned with arrays of microchannels and discontinuous microwalls,” Biomaterials, vol. 31, no. 24, pp. 6228–6238, 2010 Keywords: Tissue Engineering, Cell response, Biodegradable material, matrix-cell interaction Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials for cellular programming Citation: Agrawal A, Lee B, Irvine SA, Jia A, Bhuthalingam R, Singh V, Low K, Chua C and Venkatraman SS (2016). Aligned melt spun PCL fibers orientate smooth muscle cells and the preservation of the contractile phenotype. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00978 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Animesh Agrawal Bae Hoon Lee Scott A Irvine An Jia Ramya Bhuthalingam Vaishali Singh Kok Yao Low Chee Kai Chua Subbu S Venkatraman Google Animesh Agrawal Bae Hoon Lee Scott A Irvine An Jia Ramya Bhuthalingam Vaishali Singh Kok Yao Low Chee Kai Chua Subbu S Venkatraman Google Scholar Animesh Agrawal Bae Hoon Lee Scott A Irvine An Jia Ramya Bhuthalingam Vaishali Singh Kok Yao Low Chee Kai Chua Subbu S Venkatraman PubMed Animesh Agrawal Bae Hoon Lee Scott A Irvine An Jia Ramya Bhuthalingam Vaishali Singh Kok Yao Low Chee Kai Chua Subbu S Venkatraman Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.