Pursuing a novel bioactive dressing: stimulation of dermal fibroblasts with calcium-phosphate nanoparticles

Event Abstract Back to Event Pursuing a novel bioactive dressing: stimulation of dermal fibroblasts with calcium-phosphate nanoparticles Claudia Navarro-Requena1, 2, Soledad Pérez-Amodio1, 2, Óscar Castaño1, 2, 3 and Elisabeth Engel1, 2, 3 1 Institute for Bioengineering of Catalonia, Spain 2 CIBER de Bioingeniería, Biomateriales y Nanomedicina, Spain 3 Technical University of Catalonia, Materials Science and Metallurgical Engineering, Spain Introduction: Hard-to-heal wounds are a major socio-economic problem in developed countries[1]. In the recent years, bioactive dressings have been developed, mainly based in the addition of biological factors. However, these present significant limitations, such as high cost of production or regulatory challenges. Extracellular calcium could be considered as an alternative biostimulator, since it has been probed to affect the healing process of skin injuries[2],[3]. However, a better understanding of its effect is needed, together with a system to achieve a controlled release. In this study we tested whether the ionic dissolution of calcium-releasing nanoparticles achieved a similar stimulating effect as extracellular calcium on dermal fibroblast in vitro. Materials and Methods: Rat dermal fibroblasts were cultured in the presence of medium containing different calcium concentrations, normally ranging from 0.1 to 3.5mM Ca2+. Before adding it to the cells, media was incubated with calcium chloride(CaCl2) or with calcium-phosphate nanoparticles (CaP) for 24h at 37ºC and filter sterilized. Several relevant aspects in the wound healing process were assessed by different in vitro assays. Metabolic activity was determined with the Alamarblue® kit. Proliferation was measured by DNA quantification. Cell migration was quantified by a wound scratch assay. Total collagen synthesis was determined by means of a commercial kit. Matrix metalloproteinases 2 and 9 (MMP-2 and MMP-9) levels of activity were analyzed by a zymography assay. Finally, VEGF synthesis was measured with an ELISA. Results and Discussion: The effects stimulated by calcium from media conditioned with CaP did not exactly correlate with the one from CaCl2. This means that the other factors that the CaP are releasing, such as phosphates, are also affecting the cells. Interestingly, 3.5mM from both CaCl2 and CaP enhanced metabolic activity, in vitro wound closure and collagen synthesis. However, CaP decreased MMP activity, proliferation and VEGF synthesis. Conclusions: Even though calcium coming from CaP did not have the same effect as CaCl2 on dermal fibroblasts, the concentration of 3.5mM Ca2+ from CaP was able to enhance metabolic activity, migration, collagen synthesis and to decrease MMP activity. All these effects might promote a faster healing. A better understanding of how other cell types involved in the healing process respond to a range of concentrations of calcium-phosphate particles may lead to a wiser design of novel bioactive dressings for skin wound healing. FPU grant ref. AP-2012-5310; MAT2012-38793References:[1] Sen C.K. et al., 2009. Wound Repair and Regeneration, 17(6): 763–771[2] Doyle JW et al. Journal of Biomedical Materials Research. 32, 561, 1996.[3] Belmin J. et al. Journal of the American Geriatrics Society. 50, 269, 2002. Keywords: in vitro, Bioactivity, Calcium phosphate, ion release Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Biomaterials in wound healing Citation: Navarro-Requena C, Pérez-Amodio S, Castaño Ó and Engel E (2016). Pursuing a novel bioactive dressing: stimulation of dermal fibroblasts with calcium-phosphate nanoparticles. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02267 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Claudia Navarro-Requena Soledad Pérez-Amodio Óscar Castaño Elisabeth Engel Google Claudia Navarro-Requena Soledad Pérez-Amodio Óscar Castaño Elisabeth Engel Google Scholar Claudia Navarro-Requena Soledad Pérez-Amodio Óscar Castaño Elisabeth Engel PubMed Claudia Navarro-Requena Soledad Pérez-Amodio Óscar Castaño Elisabeth Engel Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.