Cell-instructive alginate-collagen hybrid gels for guided bone regeneration

Event Abstract Back to Event Cell-instructive alginate-collagen hybrid gels for guided bone regeneration Evi Lippens1, 2, 3, Manav Mehta1, 2, 3, Sidi A. Bencherif1, 2, Kangwon Lee1, 2, James C. Weaver2, Georg N. Duda2, 3 and David J. Mooney1, 2 1 Harvard University, John A. Paulson School of Engineering and Applied Sciences, United States 2 Wyss Institute for Biologically Inspired Engineering at Harvard, United States 3 Charité-Universitätsmedizin Berlin, Julius Wolff Institute, Germany The natural repair process is characterized by the recruitment of progenitor cells to the site of injury, induction of these cells to expand, and finally directing cell fate to restore tissue function. A biomaterial strategy that mimics and/or boosts this natural repair process has potential to be a successful approach for bone defect healing[1]. A hybrid scaffold based on a highly porous alginate cryogel filled with a collagen hydrogel was developed towards this end, with cell-instructive biochemical cues retained in the different material compartments. In vitro 2D cell migration, cell proliferation and osteogenic differentiation assays were first performed to screen various growth factors. These assays indicated the strong potential of PDGF-ab as a chemoattractant and mitogen for osteoprogenitors, while BMP-2 showed the strongest mineralization potential of the tested factors. The alginate composition and cryogelation temperature were subsequently optimized to obtain cryogels with aligned pores to promote cell infiltration, and tailored release kinetics. PDGF-ab was incorporated into the collagen gel component, as this led to its rapid release from the scaffold, in order to recruit host stem cells to the scaffold and induce their proliferation. BMP-2 was incorporated into the alginate cryogel component, as that led to a delayed release, in order to trigger the recruited mesenchymal stem cells to differentiate down the osteogenic lineage. In vitro cell culture experiments with rat mesenchymal stem cells and preliminary subcutaneous implantation tests confirmed colonization of the scaffold with cells within 7 days. BMP-2 release was confirmed to promote the osteogenic differentiation of cells in the scaffold at the three week time point in vitro. This functionalized hybrid scaffold is expected to ultimately enhance the different stages of tissue repair in bone healing, from cell recruitment to specific stem cell differentiation. Financial support for this study was provided from an Einstein Visiting Fellowship for DJM, funding of the Einstein Foundation Berlin through the Charité – Universitätsmedizin Berlin, Berlin-Brandenburg School for Regenerative Therapies GSC 203, an early career award for MM from the Deutsche Forschungsgemeinschaft-Nachwuchsakademie and support from the NIH (R01 DE013033).References:[1] M. Mehta et al. “Biomaterial delivery of morphogens to mimic the natural healing cascade in bone”, Adv Drug Deliv Rev. Vol. 64, Sep. 2012 Keywords: Bone Regeneration, 3D scaffold, in vivo tissue engineering, instructive microenvironment Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Regenerative medicine: biomaterials for control of tissue induction Citation: Lippens E, Mehta M, Bencherif SA, Lee K, Weaver JC, Duda GN and Mooney DJ (2016). Cell-instructive alginate-collagen hybrid gels for guided bone regeneration. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.00724 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Evi Lippens Manav Mehta Sidi A Bencherif Kangwon Lee James C Weaver Georg N Duda David J Mooney Google Evi Lippens Manav Mehta Sidi A Bencherif Kangwon Lee James C Weaver Georg N Duda David J Mooney Google Scholar Evi Lippens Manav Mehta Sidi A Bencherif Kangwon Lee James C Weaver Georg N Duda David J Mooney PubMed Evi Lippens Manav Mehta Sidi A Bencherif Kangwon Lee James C Weaver Georg N Duda David J Mooney Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.