Influence of chitosan properties on layer-by-layer films: an XPS depth-profiling investigation

Event Abstract Back to Event Influence of chitosan properties on layer-by-layer films: an XPS depth-profiling investigation Thiago B. Taketa1, Danilo M. Dos Santos2, Anderson Fiamingo2, Tonimar D. Senra2, Marisa M. Beppu1, Sérgio Paulo Campana Filho2, Robert E. Cohen3 and Michael F. Rubner4 1 University of Campinas, School of Chemical Engineering, Brazil 2 University of São Paulo, Institute of Chemistry at São Carlos, Brazil 3 Massachusetts Institute of Technology, Department of Chemical Engineering, United States 4 Massachusetts Institute of Technology, Department of Materials Science and Engineering, United States Introduction: Since chitosan is a natural polymer, its properties are dependent on many factors, including source, season, extraction and chemical reactions. In order to make chitosan a strong candidate in the polymer market, we employed a technique to convert chitin into chitosan using ultra-sound assisted deacetylation (USAD) method. The USAD method provides chitosan with controlled molecular weight (Mw) and degree of acetylation (DA), two crucial factors to the properties and applications of chitosan, and these characteristics were determined by the appropriate adjustment of the process parameters (e.g. temperature, irradiation amplitude and duration of ultrasound). Materials and Methods: The USAD method was described previously[1]. Multilayered nanofilms were assembled using the layer-by-layer (LbL) technique, in which the interaction among macromolecules with opposite electrostatic charges is, in most cases, the primary phenomenon responsible for the formation of multilayers. Films were characterized by profilometry (thickness), atomic force microscopy (roughness) and X-ray photoelectron spectroscopy (XPS) with depth profiling (superficial and internal composition)[2],[3]. Chitosan was paired with carboxymethyl cellulose (CMC) and sulfonated polystyrene (SPS). The methodology is summarized in Figure 1. Figure 1. Summary of the methodology used for this work. (A) LbL films preparation. (B) Film characterizations. Results and Discussion: CMC/CHI systems presented thicker and rougher films compared to SPS/CHI´s. The SPS/CHI systems are highly dependable on the chitosan DA, due to the electrostatic interactions that are necessary to build up the film. SPS/CHI is a system in which the fundamental driving force for the formation of the films originates from the electrostatic interaction between strongly charged species. Thus, the degree of acetylation of chitosan is going to play a major role in the film formation, as verified by XPS. For the CMC/CHI films, the non-electrostatic interactions play a major role on the multilayer formation. Since CMC is a weak polyelectrolyte, the pH of the solutions is the key factor to control the film deposition. When chitosan had a low molecular weight, its diffusion was favoured into the bulk of the films for the CMC/CHI systems. Conclusions: Changes in chitosan architecture, polyanion pair and pH can be used to molecularly control chemical and structural properties of nanostructured coatings, thus opening up new possibilities and adapting them for the desired application (e.g. drug release and antimicrobial activity). The main contribution of this work is to provide a better understanding of the effect of the architecture of chitosan and interactions with other macromolecules in layer-by-layer films. São Paulo Research Foundation (grant #13-05135-1); National Council for Scientific and Technological Development (Process 202523/2014-4); Elisabeth ShawReferences:[1] Delezuk, J. A. d. M.; Cardoso, M. B.; Domard, A.; Campana‐Filho, S. P. Polymer International 2011, 60, (6), 903-909.[2] Gilbert, J. B.; Rubner, M. F.; Cohen, R. E. Proceedings of the National Academy of Sciences 2013, 110, (17), 6651-6656.[3] Lee, H.; Gilbert, J. B.; Angilè, F. E.; Yang, R.; Lee, D.; Rubner, M. F.; Cohen, R. E. ACS Applied Materials & Interfaces 2015, 7, (1), 1004-1011. Keywords: self-assembly, biomaterial, polymer, biomacromolecule Conference: 10th World Biomaterials Congress, Montréal, Canada, 17 May - 22 May, 2016. Presentation Type: Poster Topic: Surface and interfacial characterization Citation: Taketa TB, Dos Santos DM, Fiamingo A, Senra TD, Beppu MM, Campana Filho S, Cohen RE and Rubner MF (2016). Influence of chitosan properties on layer-by-layer films: an XPS depth-profiling investigation. Front. Bioeng. Biotechnol. Conference Abstract: 10th World Biomaterials Congress. doi: 10.3389/conf.FBIOE.2016.01.02712 Copyright: The abstracts in this collection have not been subject to any Frontiers peer review or checks, and are not endorsed by Frontiers. They are made available through the Frontiers publishing platform as a service to conference organizers and presenters. The copyright in the individual abstracts is owned by the author of each abstract or his/her employer unless otherwise stated. Each abstract, as well as the collection of abstracts, are published under a Creative Commons CC-BY 4.0 (attribution) licence (https://creativecommons.org/licenses/by/4.0/) and may thus be reproduced, translated, adapted and be the subject of derivative works provided the authors and Frontiers are attributed. For Frontiers’ terms and conditions please see https://www.frontiersin.org/legal/terms-and-conditions. Received: 27 Mar 2016; Published Online: 30 Mar 2016. Login Required This action requires you to be registered with Frontiers and logged in. To register or login click here. Abstract Info Abstract The Authors in Frontiers Thiago B Taketa Danilo M Dos Santos Anderson Fiamingo Tonimar D Senra Marisa M Beppu Sérgio Paulo Campana Filho Robert E Cohen Michael F Rubner Google Thiago B Taketa Danilo M Dos Santos Anderson Fiamingo Tonimar D Senra Marisa M Beppu Sérgio Paulo Campana Filho Robert E Cohen Michael F Rubner Google Scholar Thiago B Taketa Danilo M Dos Santos Anderson Fiamingo Tonimar D Senra Marisa M Beppu Sérgio Paulo Campana Filho Robert E Cohen Michael F Rubner PubMed Thiago B Taketa Danilo M Dos Santos Anderson Fiamingo Tonimar D Senra Marisa M Beppu Sérgio Paulo Campana Filho Robert E Cohen Michael F Rubner Related Article in Frontiers Google Scholar PubMed Abstract Close Back to top Javascript is disabled. Please enable Javascript in your browser settings in order to see all the content on this page.