Genetic purity and diversity in isoenzymePeroxidase of pearl millet hybrids [Pennisetum glaucum(L.) Br. R.]

The prospective of Peroxidase Isozyme biochemical marker, have been investigation in the present study, ten pearl millet hybrids [ Pennisetum glaucum (L.) R. Br.] Genotypes were used to examine the suitability of peroxidase enzymes for characterization of pearl millet genotypes. Band number 1 (Rm = 0.120) was present in three hybrids i.e. GHB-526, GHB-538 and GHB-719. Band number 2 (0.158) was also present in three hybrids i.e. GHB-538, GHB-577 and GHB-719. Band number 3 (Rm = 0.380) was present in only one hybrid i.e. GHB-719 while band number 4 (Rm = 0.429) was present in five hybrids GHB-538, GHB-719, GHB-732, GHB-235 and GHB-757. All the seven bands were present in only one hybrid GHB-719. 9 DAG stage was found to be more effective as compared to 3 DAG and 6 DAG by showing more number of bands. Among the 10 genotypes studied, two could be differentiated from each other Total 13 bands of peroxidase isozymes were observed at 3, 6 and 9 day after germination (DAG). A total of 15 alleles were generated by isozymes at different DAG (3, 6 and 9 days) with an average of 5 bands per day. Banding pattern at 6 DAG showed that band number1, 2 and 3 (Rm = 0.525, 0.573 and 0.600) were present in all the hybrids except for GHB-744, in band number 1. At 9 DAG seven bands of peroxidase isozymes were observed having Rm value of 0.120, 0.158, 0.380, 0.429, 0.430, 0.478, and 0.760.