Phenotype Comparison of Young and Old Mouse Model of Asthma and Possible Mechanisms

SESSION TITLE: Asthma IV SESSION TYPE: Original Investigation Poster PRESENTED ON: Saturday, April 16, 2016 at 11:45 AM - 12:45 PM PURPOSE: To compare the difference between the young and old asthma phenotype in mouse models and explore the possible mechanisms. METHODS: BALB/c mice were randomly divided into a young control group, old control group, young experimental group, old experimental group. Mice were sensitized on days 1 and 2, chanllenged on days 18 and 19, on 21st day to assess pulmonary function to test airway hyperresponsiveness, count in bronchoalveolar lavage fluid (BALF) total cell and categories cell, lung tissue HE staining, immunohistochemistry observe neutrophils, eosinophils, IL-4, IL-17, MBD2 infiltration. RESULTS: Airway hyperresponsiveness was more significantly in the Young experimental group compared with old experimental group (P<0.05); The total number of BALF cell increased significantly in old experimental group compared with young experimental group (P<0.001), neutrophils increased more significantly (P<0.05); Lung inflammatory infiltration, neutrophil infiltration, IL-17 expression was increased significantly in old experimental group compared with young experimental group (P<0.01); Lung MBD2 expression was increased significantly in old experimental group compared with young experimental group (P<0.05). CONCLUSIONS: Young asthmatic mice with Th2-type immune response, eosinophil infiltration, old asthmatic mice with Th17 cell activation and mixed eosinophils and neutrophils infiltration;MBD2 involved in the pathogenesis of asthma, especially the pathogenesis of elderly asthma pathogenesis. CLINICAL IMPLICATIONS: MBD2 may influence the CD4 + T cell differentiation and function involved in the pathogenesis of asthma. DISCLOSURE: The following authors have nothing to disclose: Wenjin Sun, Xudong Xiang No Product/Research Disclosure Information