Monitoring the introgression of E genome chromosomes into triticale using multicolor GISH

Hybrids were made between triticale (AABBRR) line T182 and a Thinopyrum amphiploid 8801(AABBEE) and progenies advanced up to F-6. Multicolor genomic in situ hybridization (McGISH) revealed different ratios of R and E genome chromosomes in each of the lines examined. R and E genome chromosomes were eliminated by F-5 in two of the lines studied. The ratios of R:E genome chromosomes varied from 2:8 to 12:2 in other F-6 lines. Translocations between R and E genome chromosomes were detected as early as F-3. A single wheat/R translocation was detected in one line in F-6.