Optimization of protein extraction from fern frond using Response Surface Methodology (RSM)

Protein extractability from Nephrolepis biserrata and Arthropteris orientalis was studied under various conditions. The extraction of fern protein was conducted using alkaline, alcohol and saline treatments under various conditions of treatment concentrations and time of agitation. Central composite design of Response Surface Methodology (RSM) was used for identification of the best condition and extraction yield optimization. Results showed that alkaline treatment produced the highest extraction yield with maximum protein recovery of 5.11 mg/mL and 2.03 mg/mL for Nephrolepis biserrata and Arthropteris orientalis respectively at 0.1 M NaOH concentration and 30 min of agitation time. Keywords : Fern, Nephrolepis biserrata, Arthropteris orientalis, Response Surface Methodology, Agitation Time, Solvents Concentration.