Scalable chromatography-based purification of virus-like particle carrier for epitope based influenza A vaccine produced in Escherichia coli

•Purification of recombinant virus-like particle-based influenza A vaccine produced in Escherichia coli.•Purification based on multimodal size-exclusion chromatography medium in combination with detergent extraction and size-exclusion polishing.•The results provide an industry compatible strategy to purify virus-like particles based vaccines.