Mass spectrometric characterization of a two-glycosyltransferase tandem reaction for assembly of tetrasaccharide repeating unit of Escherichia coli O77 O-antigen.

Dawei Zhou,Chao Chen,Lingling Xu,Natalia Utkina,Leonid L. Danilov,V. I. Torgov,V. V. Veselovsky, Bin Liu,Lu Feng

Carbohydrate Research（2016）

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摘要

•MS provides valuable information about in vitro characterization of glycosyltransferases.•MS is highly valuable for precise structure elucidation of lipooligosaccharides.•The wbaD gene and wbaC gene from E. coli O77 encoding mannosyltransferases were characterized.•This study leads to complete characterization of O77 O-repeating unit synthetic pathway.

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关键词

E. coli O77,O-antigen repeating unit,Glycosyltransferases,WbaD,WbaC,Electrospray ionization multistage tandem mass spectrometry

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