Clinical evaluation of a single-reaction real-time RT-PCR for pan-dengue and chikungunya virus detection
Journal of Clinical Virology(2016)
摘要
•DENV and CHIKV co-circulate and result in overlapping clinical presentations.•A single-reaction pan-DENV–CHIKV rRT-PCR was designed.•The pan-DENV–CHIKV rRT-PCR was evaluated using serum samples from Nicaragua.•The pan-DENV–CHIKV rRT-PCR proved as sensitive as individual reference tests.•This DENV–CHIKV multiplex test may reduce costs and improve molecular workflow.
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关键词
Dengue virus,Chikungunya virus,Molecular diagnostics,Multiplex real-time RT-PCR,Background
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