The evolutionary position of Lestoniidae revealed by molecular autapomorphies in the secondary structure of rRNA besides phylogenetic reconstruction (Insecta: Hemiptera: Heteroptera)

Pentatomoidea (stink bugs and their relatives) is the third largest superfamily in Heteroptera, or the true bugs. The phylogenetic relationships among the families within Pentatomoidea remain controversial. The family Lestoniidae is morphologically highly specialized, currently including only two species endemic to Australia. Previous researchers have suggested a close relationship of Lestoniidae to either Plataspidae or Acanthosomatidae, based on morphological characters. In this study, phylogenetic tree reconstruction revealed that Lestoniidae and Acanthosomatidae form a monophyletic clade. In addition, in comparisons of the secondary structures of 18S and 28S rRNAs representing 15 families of Pentatomoidea, four length-variable regions in 18S and 28S rRNAs that can serve as autapomorphies for the clade Lestoniidae + Acanthosomatidae were recognized. Among them, E in 18S rRNA and D3-1 and D5-1 in 28S rRNA are unique in length in Lestoniidae and Acanthosomatidae. Based on the new molecular evidence and morphological evidence published by previous authors, Lestoniidae is suggested to be a highly specialized group derived from a common ancestor with Acanthosomatidae. (C) 2016 The Linnean Society of London