Cloning and Expression of A Novel Gene Encoded Thermostable Archaeal Aldolase Class II from Natural Sample

2+ binding, polypeptide binding, and active sites as other aldolaseu0027s. Phylogenetic analysis of the enzyme showed that the enzyme makes a different branch closed to ribulose-5-phosphate 4-epimerase of unculcured Acidilobus sp. The gene was expressed in E coli as a host, and produced 26kDa of protein. Further analysis of the enzyme showed that the enzyme is thermostable. In addition, the enzymewas purified through Ion Metal Affinity Chromatography and it showed as single band with the homogeneity at around 96%.