Design of chromogenic probes for efficient screening and evaluation of feruloyl esterase-like activities
Journal of Molecular Catalysis B: Enzymatic(2016)
摘要
•Hydroxycinnamate-based chromogenic substrates were synthesized in good yields.•Detection of esterase activity was achieved in both solid and liquid media.•Indolyl- and 4NTC-based synthetic probes are suitable for high-throughput screening.•4NTC ferulate is a new tool for the characterization of feruloyl esterases.
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关键词
BI-Co,CinnAE,CSA,DIPC,DMAP,FAE,GH,HTS,4NTC,4NTC-Fe,X-Fe
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