高效液相色谱荧光检测法测定豆芽中尿素含量
Food Research and Development(2014)
Abstract
建立了高效液相色谱荧光检测法测定豆芽中尿素的方法。样品用乙醇提取,提取液在酸性条件下用呫吨氢醇衍生,衍生产物经Agilent Eclipse XDB-C18柱(150 mm ×4.6 mm,5μm)分离,乙腈和0.02 mol/L乙酸钠溶液为流动相,梯度洗脱,荧光检测器检测波长λex 213 nm和λem 308 nm。结果表明:尿素的质量浓度在1.0 mg/L~50 mg/L范围内,峰面积与质量浓度呈良好的线性关系,r2大于0.999,方法检出限为(S/N=3)0.5 mg/kg;尿素添加浓度为5、25、50 mg/kg时,加标回收率98.5%~106%,RSD%小于4%。采用该方法检测实验室自发豆芽、绿色食品标签豆芽和市售散装豆芽(绿豆芽和黄豆芽)尿素含量不等,浓度范围分别在3.5 mg/kg~14.1 mg/kg(n=6),5.7 mg/kg~10.4 mg/kg(n=4)和7.6 mg/kg~148 mg/kg (n=35)。该方法简便、准确、灵敏、专一性强、重现性好。
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Key words
soybean sprouts,urea residue,liquid chromatography,high-performance
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