Streptococcus Chenjunshii Sp. Nov. Isolated From Feces Of Tibetan Antelopes
INTERNATIONAL JOURNAL OF SYSTEMATIC AND EVOLUTIONARY MICROBIOLOGY(2019)
摘要
Three Gram-stain-positive, catalase-negative, a-haemolytic, chain-forming and coccus-shaped microorganisms (strains Z15(T), Z1 and Z2) were isolated from feces of Tibetan antelopes collected from the Qinghai-Tibet plateau, PR China. The results of 16S rRNA gene sequence studies indicated that Z15(T) shared 94.5, 93.1 and 92.2 % similarity with Streptococcus pantholopis DSM 102135(T), Streptococcus ursoris NUM 1615(T) and Streptococcus dentapri NUM 1529(T), respectively. rpoB and groEL-based sequence analysis of our three novel isolates revealed interspecies divergence of 16.7 and 14.3 % from Streptococcus pantholopis DSM 102135(T). The genomic DNA G+C content of Z15(T) is 42.48 mol%. Z15(T) has an average nucleotide identity (ANI) value of 81.19 % with S. pantholopis DSM 102135(T) and a DNA-DNA relatedness value of less than 70 % in the in-silico DNA-DNA hybridization (isDDH) with other species of genus Streptococcus deposited in the GenBank database. A wholegenome phylogenetic tree based on 246 core genes of 78 genomes of members of the genus Streptococcus indicated that Z15(T) represents a member of genus Streptococcus but one well separated from the currently recognized species. Z15(T) contains C-18(:1)omega 7c (25.5 %), C-18:(1)omega 9c (19.6 %),C-1(6:0) (1 7.5 %) and C-16(:1)omega 9c (13.3 %) as its major cellular fatty acids. According to the morphological, biochemical and molecular phylogenetic features of the three novel isolates, they represent a novel species of the genus Streptococcus, and Streptococcus chenjunshii sp. nov. is thus proposed. The type strain is Z15(T) (=CGMCC 1.16529=DSM 106182).
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关键词
Streptococcus chenjunshii, Tibetan antelopes, 16S rRNA, isDDH, Qinghai-Tibet Plateau, ANI
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