Trimethine Cyanine Dyes As Deep-Red Fluorescent Indicators With High Selectivity To The Internal Loop Of The Bacterial A-Site Rna

We report that TO-PRO-3, a thiazole orange analogue with a trimethine bridge, functions as a deep-red fluorescent indicator for the internal loop structure of the bacterial (Escherichia coli) ribosomal decoding region of the aminoacyl-tRNA site (A-site), which enables the assessment of A-site binding capability of various test compounds including blue and even-green-emitting compounds.