Performance Characteristics And Clinical Value Of The Lipoprotein-Associated Phospholipase A(2) By An Enzymatic Kinetic Method

Objective: To analyze the performance characteristics, stability, and clinical value of lipoprotein-associated phospholipase A(2) (Lp-PLA(2)) using an enzymatic kinetic method.Methods: The performance characteristics included reference intervals, precision, and accuracy. We assessed Lp-PLA(2) stability by comparing Lp-PLA(2) changes under different conditions. Lp-PLA(2) was determined in the following groups: control individuals, patients with coronary heart disease (CHD), patients of different lipid subgroups within CHD, and patients with high total cholesterol (TC). Also, correlations between Lp-PLA(2) and traditional cardiovascular risk factors were assessed.Results: The mean (SD) reference interval of serum Lp-PLA(2) activity was 451 (113) U per L with sex differences. Inter- and intra-assay precision revealed coefficients of variance (CVs) of 1.81% to 2.63% and 1.43% to 1.77%. The average bias was 0.33%. Lp-PLA(2) activity was stable. In the CHD group, high-lipid subgroups, and high-TC group, Lp-PLA(2) was elevated, and correlation was observed between Lp-PLA(2) and traditional risk factors.Conclusion: Lp-PLA(2) activity has important clinical value in CHD.