Bacillus Salacetis Sp. Nov., A Slightly Halophilic Bacterium From Thai Shrimp Paste (Ka-Pi)

A Gram-stain-positive, slightly halophilic, endospore-forming, strictly aerobic, rod-shaped bacterium, designated SKP7-4(T), was isolated from shrimp paste collected from Samut Sakhon province, Thailand. Strain SKP7-4(T)- grew at pH 6.0-9.0 (optimum, 7.5), at 20-40 degrees C (37 degrees C) and in 0-15 % (w/v) NaCl (1-3 %). The diamino was meso-diaminopimelic acid. Menaquinone with seven isoprene units Diphosphatidylglycerol, phosphatidylglycerol, phosphatidylethanolamine and three as polar lipids. It contained iso-C-15.0 and anteisoC(15)(.0) as major cellular fatty acid found in the cell-wall peptidoglycan was the major isoprenoid quinone. unidentified phospholipids were detected acids. On the basis of 16S rRNA gene sequence analysis, strain SKP7-4(T)- belonged to the genus Bacillus and was closely related to Bacillus vietnamensis JCM 11124(T), Bacillus marisflavi JCM 11544(T), Bacillus aquimaris JCM 11545(T) and Bacillus oryzaecorticis JCM 19602(T), with 98.7, 97.9, 97.8 and 97.8 % similarity, respectively. The draft genome of SKP7-4(T)- was 4.68 Mb with 5208 coding sequences with an average G+C content of 43.2 mol%. The ANIb and ANIm values of strain SKP7-4(T)- were 70.0 and 84.3 %, respectively, and the digital DNA-DNA hybridization value was 20 % in comparison with the draft genome of B. vietnamensis JCM 11124(T). On the basis of the results of phenotypic, chemotaxonomic and phylogenetic analyses, the strain should represent a novel species of the genus Bacillus and the name Bacillus salacetis sp. nov. is proposed. The type strain is SKP7-4(T)- (=JCM 33205(T) =KCTC 43014(T) =TISTR 2596(T)).