The transcription factor Duxbl mediates elimination of pre-T cells that fail β-selection.

T cell development is critically dependent on successful rearrangement of antigen-receptor chains. At the beta-selection checkpoint, only cells with a functional rearrangement continue in development. However, how nonselected T cells proceed in their dead-end fate is not clear. We identified low CD27 expression to mark pre-T cells that have failed to rearrange their beta-chain. Expression profiling and single-cell transcriptome clustering identified a developmental trajectory through beta-selection and revealed specific expression of the transcription factor Duxbl at a stage of high recombination activity before beta-selection. Conditional transgenic expression of Duxbl resulted in a developmental block at the DN3-to-DN4 transition due to reduced proliferation and enhanced apoptosis, whereas RNA silencing of Duxbl led to a decrease in apoptosis. Transcriptome analysis linked Duxbl to elevated expression of the apoptosis-inducing Oas/RNaseL pathway. RNaseL deficiency or sustained Bcl2 expression led to a partial rescue of cells in Duxbl transgenic mice. These findings identify Duxbl as a regulator of beta-selection by inducing apoptosis in cells with a nonfunctional rearrangement.