Resistance gene cloning from a wild crop relative by sequence capture and association genetics
NATURE BIOTECHNOLOGY(2019)
Abstract
Disease resistance (R) genes from wild relatives could be used to engineer broad-spectrum resistance in domesticated crops. We combined association genetics with R gene enrichment sequencing (AgRenSeq) to exploit pan-genome variation in wild diploid wheat and rapidly clone four stem rust resistance genes. AgRenSeq enables R gene cloning in any crop that has a diverse germplasm panel.
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Key words
Agricultural genetics,Plant biotechnology,Plant molecular biology,Life Sciences,general,Biotechnology,Biomedicine,Agriculture,Biomedical Engineering/Biotechnology,Bioinformatics
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