[Optimization of chromosome flaking technique for mesenchymal stem cells].
Zhonghua yi xue yi chuan xue za zhi = Zhonghua yixue yichuanxue zazhi = Chinese journal of medical genetics(2019)
Abstract
OBJECTIVE:To optimize the condition for chromosome flaking of mesenchymal stem cells to ensure the cytogenetic quality control of expanding production and clinical application.
METHODS:Chromosomal flaking methods were optimized from current chromosome preparation techniques from the aspects of MSCs cell culture concentration, colchicine treatment time and low permeability time.
RESULTS:By repeated pre-experiments, the optimal MSCS chromosome flaking condition of MSCs was determined as cell culture concentration of (1-2)× 106 cells per T25 cell culture bottle, and the colchicines processing time was determined as 2 hours and 10 minutes, and the low permeability was 1 hour.
CONCLUSION:The optimized chromosome flaking condition can fulfill the requirement of cytogenetic quality control for MSCs.
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Key words
Mesenchymal stem cell,Cytogenetic quality control,Chromosome flaking,Karyotype analysis
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