Cellulomonas Rhizosphaerae Sp. Nov., A Novel Actinomycete Isolated From Soil

A novel actinomycete, designated strain NEAU-TCZ24(T), was isolated from soil and characterized using a polyphasic approach. The results of phylogenetic analysis based on the 16S rRNA gene sequence indicated that the organism should be assigned to the genus Cellulomonas and formed a stable Glade with its closest relatives Cellulomonas terrae JCM 14899(T) (98.4 % 16S rRNA gene sequence similarity), Cellulomonas xylanilytica JCM 14281 1 (97.9 %) and Cellulomonas humilata JCM 11945(T) (97.7 %). The major menaquinones were identified as MK-9(H-4) and MK-8(H-4). The phospholipid profile was found to contain diphosphatidylglycerol, phosphatidylethanolamine, phosphatidylglycerol, phosphatidylinositolmannoside, a ninhydrinpositiveglycolipid, an unidentified phosphoglycolipid, an unidentified phospholipid and an unidentified lipid. The major fatty acids were identified as anteiso-C-15:0,C-18:1 omega 9c, C-16:0 and anteiso-C-17:0. Moreover, morphological and chemotaxonomic properties of NEAU-TCZ24(T) also confirmed the affiliation of the isolate to the genus Cellulomonas. However, multilocus sequence analysis based on five other house-keeping genes (gyrB, rpoB, recA, relA and atpD), DNA-DNA relatedness, physiological and biochemical data indicated that NEAU-TCZ24(T) could be distinguished from its closest relatives. Therefore, it is proposed that NEAU-TCZ24(T) represents a novel species of the genus Cellulomonas, for which the name Cellulomonas rhizosphaerae sp. nov. is proposed. The type strain is NEAU-TCZ24(T) (=CCTCC AA 2018042(T)=JCM 32383(T)).