Motilimonas Pumila Sp. Nov., Isolated From The Gut Of Sea Cucumber Apostichopus Japonicus

A facultatively anaerobic and Gram-negative bacterium, designated strain PLHSC7-2(T), was isolated from the gut of sea cucumber Apostichopusjaponicus that had been collected from the coastal area of Yantai, China. The cells were rod-shaped and motile by means of polar flagella. The novel isolate grew optimally at 28-30 degrees C, in the presence of 2.0-3.0 % (w/v) NaCl and at pH 7.0-7.5. The sole respiratory quinone was Q-8 and the major fatty acids were summed feature 3(C-16 : 1 omega 7c and/or C-16 : 1 omega 6c), C-16 : 0 and C-17 : 0. The predominant polar lipids were phosphatidylethanolamine, phosphatidylglycerol and diphosphatidylglycerol. Phylogenetic analyses based on 16S rRNA gene sequences showed that strain PLHSC7-2(T) was phylogenetically affiliated with the genus Motilimonas, and exhibited sequence similarity of 96.2 % toMotilimonaseburnea YH6(T). The DNA G+C content was 45.5 mol%. On the basis of phenotypic , phylogenetic and genetic distinctiveness, strain PLHSC7-2(T)(=MCCC 1 K03522(T) =KCTC 62589(T)) was classified as a novel species within the genus Motilimonas, for which the name Motilimonas pumila sp. nov. is proposed.