Estrogen sensitive liver transgenic zebrafish (Danio rerio) line (Tg(vtg1:mCherry)) suitable for the direct detection of estrogenicity in environmental samples.

Katalin Bakos,Robert Kovacs, Erna Balogh,Dora Kanaine Sipos,Marta Reining, Orsolya Gyomorei-Neuberger,Adrienn Balazs,Balazs Kriszt,Dora Bencsik, Andrea Csepeli,Gyongyi Gazsi,Yavor Hadzhiev,Bela Urbanyi,Ferenc Mueller,Balazs Kovacs,Zsolt Csenki

Aquatic Toxicology（2019）

Cited 18|Views27

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Abstract

•Tg(vtg1:mCherry) expresses mCherry under the control of a 3357 bp promoter of the zebrafish vtg1and carries 26 natural EREs.•Endogenous vtg and mCherry are produced only in the left hepatic lobe of 5 dpf zebrafish embryos and show similar spatial expression.•Tg(vtg1:mCherry) embryos detect E2 from 100 ng/L, EE2 from 1 ng/L, ZEA from 100 ng/L and BPA from 1 mg/L.•Tg(vtg1:mCherry) adults are more sensitive, with detectable transgene activity from 5 ng/L E2.•Tg(vtg1:mCherry) is suitable for the direct measurement of estrogenicity in wastewater samples without sample extraction.

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Key words

Transgenic,Vitellogenin,Endocrine disrupting chemicals,In vivo,YES

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