Purification of Functional F-ATP Synthase from Blue Native PAGE.
CALCIUM SIGNALLING: METHODS AND PROTOCOLS(2019)
摘要
In the presence of Ca, F-ATP synthase preparations eluted from Blue Native gels generate electrophysiological currents that are typical of an inner mitochondrial membrane mega-channel, the permeability transition pore. Here we describe an experimental protocol for purification of F-ATP synthase that allows to maintain the enzyme assembly and activity that are essential for catalysis and channel formation.
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关键词
Blue Native PAGE,F-ATP synthase,Gel purification,Lipid bilayer,Permeability transition
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