TaZFP1, a C2H2 type-ZFP gene of T. aestivum, mediates salt stress tolerance of plants by modulating diverse stress-defensive physiological processes.

Salt stress suppresses plant growth, development, and crop productivity. In this study, we characterized the role of TaZFP1, a C2H2 type-zinc finger protein family member of T. aestivum, in salt stress tolerance. TaZFP1 possesses a conserved C2H2 motif (CX2-4CX12HX3-5H) shared by plant ZFP proteins, translocates to the nucleus after endoplasmic reticulum (ER) assortment, and displays a ZF 3-D structure similar to its eukaryote homologs. The transcripts of TaZFP1 were upregulated during salt stress condition and this effect was restored under normal conditions. Compared to wild type (WT), the transgenic lines of TaZFP1 overexpression or knockdown displayed improved phenotypes, biomass, photosynthesis parameters (Pn, ΨPSII, and NPQ), osmolytes contents (i.e. proline and soluble sugar), and enhanced antioxidant enzyme (AE) activity following salt stress treatment. A set of genes associated with proline synthesis (i.e., NtP5CS1 and NtP5CS2) and encoding AEs (i.e., NtSOD2, NtCAT1, and NtPOD4) were upregulated in the salt-challenged transgenic lines of TaZFP1 expression. Additionally, the transgenic lines exhibited similar stomata movement patterns and leaf water retention properties under salinity conditions compared to those induced by exogenous abscisic acid (ABA) treatment, suggesting that the TaZFP1-mediated salt response is dependent on the ABA signaling. High throughput RNAseq analysis revealed significant alteration of gene transcription in transgenic lines upon salt stress. Among them, the differentially expressed genes (DEGs) represented by the gene ontology (GO) terms were associated with organic acid, carboxylic acid, carbohydrate, and coenzyme as well as organonitrogen compounds, translation, peptide metabolism, and peptide biosynthesis. A set of upregulated DEGs were found to be thylakoid- and photosystem-associated, which is consistent with the TaZFP1-mediated improvement in photosynthesis in salt-stressed transgenic lines. Our investigation indicated that the TaZFP1-mediated salt tolerance is ascribed to the regulation of gene functions related to photosynthesis, osmolytes metabolism and ROS homeostasis mediated by ABA signaling.