Extraction and Characterization of Secondary Metabolites from the Soil Bacterium, Acidovorax sp. SA5 and Evaluation of Their Larvicidal Activity Against Aedes aegypti

The present investigation deals with the extracellular metabolites from the soil bacterium against the devastating vector , Aedes aegypti . The testing of cell-free supernatant (CFS) of the soil bacterium, SA5 against third instar larvae of A. aegypti revealed LC 50 and LC 90 values (µL/mL) of 45.43 and 77.77, respectively. Histopathological analysis of third instar larvae of A. aegypti treated with CFS (1500 µL) after 24 h showed highly damaged epithelial cells, midgut and cortex regions. The brine shrimp toxicity of the effective CFS of SA5 strain on Artemia nauplii , exhibited only low lethality effect (33%). The bacterial genomic DNA of the effective SA5 strain was found to possess 1330 bp and was identified as Acidovorax sp. strain (MF581272) through molecular characterization. The FTIR, HPLC and GC–MS analyses of the CFS of Acidovorax sp. revealed 18 functional groups, 12 peaks and 10 compounds, respectively. The SDS-PAGE analysis of the extracellular protein from the CFS revealed their molecular weight range as 15–165 kDa. In view of the rerecorded potent anti-mosquitocidal property of the metabolites of the Acidovorax sp., further studies on their active principles are warranted.